Since few transplanted cells persist in vitro, the beneficial effects of cell therapy may lie in the secreted factors being the active component of this treatment. A key part of paracrine secretion is Exosomes, which are membrane vesicles that are stored intracellularly in endosomal compartments and are secreted when these structures fuse with the cell plasma membrane.

• 1.- Exosomes contain protein, DNA, and RNA, thus making them an attractive vector of paracrine signals delivered by stem cells.
• 2.- Exosomes may also be "loaded" with predetermined proteins and nucleic acid to achieve a desired effect (1).
• 3.- Exosomes can be stored as an "off-the-shelf" product having the potential for circumventing many of the limitations of viable cells for therapeutic applications in regenerative medicine.
• 4.- In vitro, exosomes from pre-adipocytes stimulate cell proliferation in a wound healing model.
• 5.- In vitro, adipose-graft derived exosomes have been shown to be a promising tool for skin repair and remodeling (2)

Quality Control: All cells have been screened negative for HIV-1, HIV-2, Hep-B, Hep-C and Syphilis. All isolated and concentrated exosomes undergo includes Particle mean diameter, Protein concentration, RNA concentration and Concentration of particles/ml.